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Mains of TRAF3 and TRAF5 is also incapable of NF-kB activation. The authors proposed that these chimeric proteins might not be able to chelate zinc ions or fold properly. This may explain why AN2R is unable to bind any Cullin. However, the other Roc chimeras we constructed were able to bind Cullin and were active as E3 ligases. Moreover, the rescue of male sterility by the ANBR chimera (see below)
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