Antibody. doi:10.1371/journal.pone.0002918.gdently of other Roc-Cullin complexes and that mutations of each gene would cause the same phenotype. In addition, this hypothesis predicts that the Roc2 mutant phenotype would be different than the phenotype we previously determined for Roc1a and Roc1b mutants [24,25]. The Roc2 locus is complex, with two protein coding exons separated by an intron greate

Antibody. doi:10.1371/journal.pone.0002918.gdently of other Roc-Cullin complexes and that mutations of each gene would cause the same phenotype. In addition, this hypothesis predicts that the Roc2 mutant phenotype would be different than the phenotype we previously determined for Roc1a and Roc1b mutants [24,25]. The Roc2 locus is complex, with two protein coding exons separated by an intron greate

Massie stained gel of purified GST-Roc proteins used in the ligase assays. Bracket indicates GST-Roc proteins. Asterisk indicates GST. Lower bands are degradation products. B, All Drosophila Roc proteins and chimeras were assessed for ligase activity in a substrate free assay. Briefly, 250 ng of Roc protein (or control GST) were added to a ubiquitin ligase mixture containing UbcH5, ubiquitin, and

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